1) BACTERICIDAL ACTIVITY (TESTS CARRIED OUT IN EUROPE)
Testing the neutral anolyte against two bacterial isolates from the uterus of mares.
a ) - Streptococcus zooepidemicus
The microbial activity of the neutral anolyte was examined against methicillin sensory: Staphylococcus Aureus, Staphylococcus epermidis, Serratia marcescens, Escherichia coli, Pseudomonas aeruginosa and Burkholderia Cepacia which are serious pathogens.
The bactericidal properties of the neutral anolyte were evaluated with three conventional disinfectants which are: Chlorhexidine at 0.1%, povidone iodine 0.02% and 80% ethanol. The concentrations chosen represent those generally used in the solutions prepared for washing hands. All disinfecting solutions were mixed with sterile distilled water at the time of their use. The sterile distilled water was used as control. The results are summarized in Tables 1 and 2.
The ANAQUA units and neutral anolyte products have been tested by large series of independent tests and on site trials, including hospitals, breweries, water treatment systems and agricultural environments.
b) - Escherichia coli
Bacterial counts
Bacterial counts
Total viable count of inoculum> 5000 cfu / ml
Total viable count of inoculum> 5000 cfu / ml
Microbiological Performance
Independent testing
Exposure Time
Dilution
30 s
1 min
5 min
10min
20 min
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
10
2
10
3
10
4
10
5
Exposure
Dilution
30 s
1 min
5 min
10min
20 min
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
10
2
10
3
10
5
10
4
Table 1. Bactericidal effect of neutral anolyte on an inoculum of 1.7 x 104 cfu/mL
Trial 1
TRIALS
MADE
ON SITES
Being environmentally friendly, the neutral anolyte is completely safe, non-toxic and non-irritating. It can be used where traditional chemicals do not produce the desired results or where traditional products cannot be used.
The findings show that the neutral anolyte is an inexpensive and powerful disinfectant with many benefits to be recommended in multiple solution, sterilization procedures, disinfection and water purification.
A safer world
The alternative solution
Trial 2
Table 2. Bactericidal effect anolyte inoculum of 1.7 x 106 cfu/mL
Bacteria
Disinfectant
10 s
60 s
180 s
Contact time
MSSA methicillin sensitive Staphylococcus aureus
MRSA methicillin resistant Staphylococcus aureus
Staphylococcus epedermis
Pseudomonas aeruginosa
Escherichia coli
Serratia marcescens
Burkholderia cepacia
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>27
>500
0
0
0
>500
>500
0
0
0
0
>500
0
0
0
0
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
41
>500
0
0
0
0
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
0
>500
0
0
0
0
>500
0
0
0
>500
>500
0
0
0
0
>500
0
0
0
0
>500
MSSA methicillin sensitive Staphylococcus aureus
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
MRSA methicillin resistant Staphylococcus aureus
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Staphylococcus epedermis
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Pseudomonas aeruginosa
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Escherichia coli
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Serratia marcescens
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Burkholderia cepacia
Neutral anolyte
0.02% Povidone iodine
80% Ethanol
0.1% chlorhexidine
Control: distilled water
Bacteria
Disinfectant
0
8
0
>500
>500
0
15
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
3
>500
0
>500
>500
0
0
0
>500
>500
0
71
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>27
>500
0
237
0
>500
>500
0
0
0
>500
>500
0
0
0
1
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
0
>500
0
0
0
>500
>500
0
0
0
>500
>500
0
0
0
0
>500
10 s
60 s
180 s
Contact time
Conclusion : The number of bacteria was reduced below the detection limit after 10 seconds incubation in neutral anolyte. The bactericidal activity of the neutral anolyte was similar to that of ethanol at 80%, but greater than 0.1% chlorhexidine and 0.02% iodine Povidone. We conclude that the anolyte neutral is a powerful, cost-effective disinfectant.
Trial 3
Microorganism
Inoculum concentration
Contact time
Escherichia coli
1, 5, 10 et 30 minutes
Salmonella sp.
1, 5, 10 et 30 minutes
Pseudomonas aeruginosa
5, 10, 30 et 60 minutes
Pneumophila legionella
5, 10, 30 et 60 minutes
1,5 x 10 cfu/ml
5
Parameter
Units
Initial
values
1 min.
5 min
10 min.
30 min.
pH
PH unit
8,1
8,1
8,1
8,1
8,0
Residual chlorine
Units
mg/l
0,7
0,7
0,7
0,7
Redox potential
mV
825
-
-
-
-
Escherichia coli
cfu / 100ml
1,5 x 10
0
0
0
0
Heterotrophic
bacteria
cfu / ml
< 1
-
-
-
-
1,3 x 10 cfu/ml
5
1,1 x 10 cfu/ml
5
1,5 x 10 cfu/ml
5
Tests of the neutral anolyte against Escherichia coli, Salmonella species, Pseudomonas aeruginosa and Legionella pneumophila
Testing Results
Table 1
Neutral anolyte + Bacteria
5
Table 2
Parameter
Units
Initial
values
1 min.
5 min
10 min.
30 min.
pH
PH unit
8,1
8,1
8,1
8,1
8,0
Residual chlorine
Units
mg/l
0,7
0,7
0,7
0,7
Redox potential
mV
825
-
-
-
-
Salmonella spp.
cfu / 100ml
1,3 x 10
NEG
NEG
NEG
NEG
Heterotrophic
bacteria
cfu / ml
< 1
-
-
-
-
Neutral anolyte + Bacteria
5
Table 3
Parameter
Units
Initial
values
1 min.
5 min
10 min.
30 min.
pH
PH unit
8,1
8,1
8,1
8,1
8,0
Residual chlorine
Units
mg/l
0,7
0,7
0,7
0,7
Redox potential
mV
825
-
-
-
-
Pseudomonas aeruginosa
cfu / 100ml
1,1 x 10
POS
NEG
NEG
NEG
Heterotrophic
bacteria
cfu / ml
< 1
150
< 1
< 1
< 1
Neutral anolyte + Bacteria
5
Table 4
Parameter
Units
Initial
values
1 min.
5 min
10 min.
30 min.
pH
PH unit
8,1
8,1
8,1
8,1
8,0
Residual chlorine
Units
mg/l
0,7
0,7
0,7
0,7
Redox potential
mV
825
-
-
-
-
Legionella
pneumophila
cfu / 100ml
1,5 x 10
1200
200
0
0
Heterotrophic
bacteria
cfu / ml
< 1
1200
200
< 1
< 1
Neutral anolyte + Bacteria
5
CONCLUSION : This study shows that the disinfecting action of the neutral anolyte is fast and efficient. It takes one minute to eradicate E. coli and Salmonella sp. and, after 10 minutes, no more of the tested strains are present.
2) SPORICIDAL ACTIVITY
Remaining spores (log10), after exposure to the neutral anolyte or 2% glutaraldehyde
|
Contact Time |
Anolyte Neutral |
|
2% glutaraldehyde |
|
|
Pre - disinfection challenge |
|
7,76 |
|
7,76 |
|
1 min |
|
4,84 |
|
7,63 |
|
2 min |
|
2,34 |
|
7,60 |
|
5 min |
|
1,30 |
|
7,46 |
|
10 min |
|
0 |
|
7,19 |
|
20 min |
|
0 |
|
6,87 |
|
30 min |
|
0 |
|
6,34 |
|
1 heure |
|
0 |
|
2,75 |
|
2 heures |
|
0 |
|
0 |
CONCLUSION : This study shows that the neutral anolyte produced using ANAQUA units is highly effective as a sporicidal agent. A reduction of 6log10 spores with a freshly produced anolyte neutral solution was observed in 5 minutes.